Data from: Diffusion tensor imaging of dolphin brains reveals direct auditory pathway to temporal lobe

The brains of odontocetes (toothed whales) look grossly different than their terrestrial relatives. Because of their adaptation to the aquatic environment and their reliance on echolocation, the odontocetes’ auditory system is both unique and crucial to their survival. Yet, scant data exist about the functional organization of the cetacean auditory system. A predominant hypothesis is that the primary auditory cortex lies in the suprasylvian gyrus along the vertex of the hemispheres, with this position induced by expansion of “associative” regions in lateral and caudal directions. However, the precise location of auditory cortex and its connections are still unknown. Here, we used a novel diffusion tensor imaging (DTI) sequence in archival postmortem brains of a common dolphin (Delphinus delphis) and a pantropical dolphin (Stenella attenuata) to map their sensory and motor systems. Using thalamic parcellation based on traditionally defined regions for primary visual (V1) and auditory cortex (A1), we found distinct regions of the thalamus connected to V1 and A1. But in addition to suprasylvian-A1, we report here, for the first time, auditory cortex also exists in the temporal lobe, in a region near cetacean-A2 and possibly analogous to primary auditory cortex in related terrestrial mammals (artiodactyla). Using probabilistic tract tracing, we found a direct pathway from inferior colliculus to medial geniculate nucleus to temporal lobe near the sylvian fissure. Our results demonstrate the feasibility of postmortem DTI in archival specimens to answer basic questions in comparative neurobiology in a way that has not previously been possible and shows a link between the cetacean auditory system and those of terrestrial mammals. Given that fresh cetacean specimens are relatively rare, the ability to measure connectivity in archival specimens opens up a plethora of possibilities for investigating neuroanatomy in cetaceans and other species.