Staphylococci Knockout Screening

Sample Genotype Detail Tryptic soy broth (TSB) Blank standard growth media control AH5011 S. hominis D11 (wildtype) Postive control / Bacteriocin-producing strain AH6424 S. hominis AH5011 ∆pHom∆pMup Plasmid-cured mutant AH0203 S. aureus RN4220 (wildtype) Negative control AH6425 S. aureus RN4220 pCM28-HomLocus Reengineered S. aureus-expressing bacteriocin AH6433 S. aureus RN4220 pCM28-empty vector Negative control / Empty vector control AH6434 S. aureus RN4220 pCM28-HomLocus-∆homI Reengineered S. aureus-expressing bacteriocin without the immunity protein, HomI AH6447 S. aureus RN4220 pCM28-HomLocus-∆homBCDM Reengineered S. aureus-expressing bacteriocin without the biosynthesis genes: HomB, HomC, HomD2, and HomM2 D11_Peptide Purified bacteriocin 1 , 2, 3 - Injection Replicates