Improving blood vessel function in peripheral artery disease: endothelial-derived TRAIL generates stable microvessels in ischemia via TRAIL-R2

Ischemia reduction in peripheral artery disease (PAD) through angiogenesis requires endothelial cell (EC) and pericyte interactions to form stable microvascular networks, but processes mediating this are disrupted in PAD. Patients with cardiovascular disease have suppressed levels of tumour necrosis factor-related apoptosis-inducing ligand (TRAIL). The contribution of endothelial cell (EC)-derived TRAIL to generating stable blood vessel networks and the impact of its receptors in PAD is unknown.We used scRNA-seq to identify molecular determinants that are altered in ischemic tissues of EC-specifc TRAIL KO mice vs littremate control mice, specifically looking at EC-pericyte interactions. Gastrocnemius muscle were collected from corneol and ishemic limbs, digested, crupreserved, and then subjected to scRNA-seq for analysis