1-deoxysphingolipids bind to COUP-TF to modulate lymphatic and cardiac cell development

We identified 1-deoxysphingosine as a ligand for NR2F1 and NR2F2. To prove physiological relevance after in vitro binding assay we investigated the role of NR2F1 and NR2F2 in cardiomyocyte differentiation. We also used different strategies to manipulate levels of 1-deoxysphingosines and canonical sphingosines in cardiomyocytes derived from human embryonic stem cells. Here we generated both single knockout of NR2F2 and double knockouts of NR2F1 and NR2F2 in human embryonic stem cell line H9. The differentiation of WT, KO and DKO lines into cardiomyocytes was then compared by single-cell RNA-seq analysis. Overall design: Examination of cell clusters in WT, NR2F2 single knockout and NR2F1 and NR2F2 double knockout embryonic stem cells during the differentiation into cardiomyocytes