9.3. Human adipose-derived Mesenchymal Stem/Stromal stemness markers and dissociation results when cultured in Hydrogel Microbeads Seeded with 1 or 2 Million cells/mL, produced with Bioink 3 via Inkjet Bioprinting Using either a 50 or 100 µm Nozzle and Cultured for 21 Days.

Stemness markers of human adipose-derived Mesenchymal Stem/Stromal cells viability when cultured in Hydrogel Microbeads Seeded with 1 or 2 Million cells/mL, produced with Bioink 3 via Inkjet Bioprinting Using either a 50 or 100 µm Nozzle and Cultured for 21 Days. Dissociation results of the cells isolated from the analysed microbeads are also provided as an excel file Inkjet bioprinting parameters: Nozzle diameter: 50 or 100 µm. Aperture time: 10 ms. Jetting pressure: 3 bar for 100 µm nozzle, 4 bar for 50 µm nozzle. Inkjet device temperature: 37°C. Seeding density: 1 million AD-MSCs/mL of bioink for 50 µm nozzle; 1 and 2 million AD-MSCs/mL of bioink for 100 µm nozzle. Sample codes: DAG 251 = HAG 251: bioink 3 with a hyaluronic acid/dermatan sulfate/collagen cocktail instead of fibrinogen. Name codes: J"1", J"2"...: days in culture since biofabrication. D"1", D"2"...: days in culture since biofabrication. 1M: cell seeding of 1 million cell/mL of bioink. 2M: cell seeding of 2 million cell/mL of bioink. NM: non-marquées (cells without labelling, used to calibrate the citometer). iso: isotype internal control to calibrate unespecific signals. viab: viability sample labelled with annexin (labels apoptotic cells) and propidium iodide (PI, labels necrotic cells). Monomarquage: samples labelled with only one marker to compensate internal citometer signals.