Microarray of Sirt1 KO and control astrocytes after cocktail stimulation

The affection of Sirt1 on astrocyte is not well understanded. Here we studied the change of RNA expression profile after Sirt1 knockout in primary astrocyte after stimulating with A1 astrocyte inducing cocktails. To analyze the change of RNA expression profile after Sirt1 knockout in astrocytes, we built Sirt1 knockout and control astrocytes using crispr technique, and analyzed RNA expression profiles of Sirt1 KO and control astrocytes after treating with cocktail (C1q, IL-1α, TNF-α) by using microarray. Primary astrocytes isolated from p2-3 pups were treated with lentivirus carrying sgScramble (control) or sgSirt1, and selected with puromycin for 3 days. 5×10^5 sgSirt1 or sgScramble treated astrocytes were seeded in a 6-well plate in astrocyte serum-free medium. The following day, cells were treated with cytokine cocktail (C1q, IL-1α, TNF-α) for 24 h. The cells were collected immediately after cocktail stimulation. RNA was extracted by using Qiagen RNeasy Plus Kits and used for microarray.