Rachel Horowitz-Scherer, Chris Woodcock (2010) CIL:758, Patiria miniata, sperm. CIL. Dataset

Phosphorus has a characteristic electron energy loss with an edge at 160 eV. The Zeiss 902 electron microscope permits images to be recorded at different energy losses which can be digitized and processed to yield a net phosphorus image. For nuclear chromatin, phosphorus is primarily located in DNA, allowing its distribution in chromatin fibers to be mapped.
Starfish sperm chromatin, which has clearly defined chromatin fibers was fixed, embedded in Lowicryl at low temperature, thin sections prepared and imaged unstained.
The 4 grouped images were recorded with a Zeiss 902 energy filtered TEM at 80KV using 5eV windows centered on energy loss values of 170, 160, 118, and 104eV (this image).

磷元素具有特征性的电子能量损失，其边缘位于160电子伏特。蔡司902型电子显微镜允许记录不同能量损失下的图像，这些图像可数字化并处理以生成净磷图像。对于核染色质，磷主要位于DNA中，这使其在染色质纤维中的分布得以映射。海星精子的染色质，其染色质纤维结构清晰可辨，经过固定、低温Lowicryl包埋，制备了薄片并进行了无染色成像。通过蔡司902型能量过滤透射电子显微镜在80KV电压下，使用以170、160、118和104电子伏特能量损失值为中心的5电子伏特窗口，记录了4组图像（此图像）。