A global transcriptome analysis of human dermal fibroblasts upon overexpression of microRNA-132. Homo sapiens

MiR-132 is one of the most upregulated miRNAs in human skin wounds at the inflammatory phase of healing; however its biological role in dermal fibroblasts during wound repair has not been studied. To study the genes regulated by miR-132, we transfected miR-132 mimics (pre-miR-132) into primary human dermal fibroblasts to overexpress miR-132. We performed a global transcriptome analysis of fibroblasts upon overexpression of miR-132 using Affymetrix arrays. Overall design: Expression profiling of primary human dermal fibroblasts transfected with 20nM miR-132 mimics (pre-miR-132) or control oligos (pre-miR-Ctrl) for 48 hours (biological triplicates in each group) was performed using Affymetrix GeneTitan system.