Transcriptome sequencing of Antheraea pernyi

The Antheraea pernyi strain Qing 6 exhibiting yellow-cyan skin color was used for transcriptome sequencing of the cuticle. The larvae were reared on Quercus wutaishanica Mayr in the field. The fifth instar larvae at day 5 were used to collect the epidermis, midgut and hemolymph. The hemolymph was first collected and immediately frozen in liquid nitrogen. The epidermis was dissected on the ice with the removal of the attached muscles and fat bodies. The isolated epidermis and midgut were washed with insect physiological saline, immediately frozen in liquid nitrogen.