Bacterial Protoplast-Derived Nanovesicles Carrying CRISPR/Cas9 Tools Efficiently Re-educate Tumor-Associated Macrophages for Cancer Immunotherapy

To investigate the activation of tumor microenvirment, we used E.coli-derived nanovesicles to treat tumor-bearing mice and detected tumor tisssues by RNA-Seq. Overall design: Comparative gene expression profiling analysis of RNA-seq data for PBS group and nanovesicle treatment group.