Transcriptomic analysis of the peripheral nervous system of Schwann cell-conditional FASN knockout mice

Myelination calls for a tremendous surge in cell metabolism towards lipids and membrane production. However, functional relevance of de novo fatty acid synthesis in myelinating cells remains unclear. We generated mutant mice in which the enzyme fatty acid synthase (FASN) was depleted conditionally in Schwann cells, the myelinating glial cells of the peripheral nervous system. To address how lack of FASN was affecting the development of the peripheral nervous system, we screened with an Affymetrix Transcriptomic approach the transcriptome of sciatic nerves and roots of P60 FASN mutant mice, comparing to control mice. N = 3 sample per condition, 2 tissues analyzed: sciatic nerve and roots, 2 genotypes: DhhCre+ Fasn lox/lox (mutant) and DhhCre- Fasn lox/lox (control)