RNAi-based GtNPR deorphanization cAMP raw values.

RIA-determined cAMP values (pM) are provided for three separate experiments (mean SEM). Treatments: C (control), Fk (Forskolin), Fk + G (Forskolin + GYIRFamide). The amount of isolated membrane differs between experiments, as evidenced by basal cAMP levels. This is in part due to differences in the size, number, and feeding behavior of worm batches used for membrane isolation. Analysis (one-way ANOVA, Tukey) of these raw datasets establishes abolishment of cAMP inhibition brought on by GYIRFamide associated with GtNPR-1 suppression. For each experimental grouping, Fk is compared to Fk + G. Asterisks indicate significance at P0.001 (***), P0.01 (**), and “ns” means no significant difference.