Characterizing the epigenome of glioma stem cells (RRBS)

Glioma initiating cells/stem cells exist in the bulk tumor of glioblastoma. This cell population contributes to the frequent resistances toward radiation/chemotherapy, aggressiveness of adult brain cancer and increased recurrence rate. Targeting stem cell population becomes one the most promising and permissive therapeutic strategies. We isolated glioma stem cells from patient-derived xenografts and profiled their epigenomic features, including 4 different DNA marks and 2 enhancer marks, and transcriptome in these in vitro cultured cell lines. Three fetal brain-derived neural stem/progenitors cells were used for comparing the unique and common molecular features in these glioma cancer stem cells. A core set of 12 glioma stem cell lines and 3 neural stem/progenitor cell lines were mapped for 5mC, 5hmC, 5fC/caC, H3K4me1, H3K27ac, and transcriptome using next generation sequencing technique.