Microarray data of human moDC treated with IFNβ and TNFα

Human moDC were treated with IFNβ and TNFα or the combination of both for either 1 h or 2.5 h in triplicate and profiled by gene expression microarray DC were treated with 4500 pg/mL TNFα, 3000 pg/mL IFNβ, or the combination of both for either 1 h or 2.5 h. Untreated DC served as a negative control. Three samples were taken per treatment and time point. RNA was extracted with the RNeasy plus kit (Qiagen) following the manufacturer’s instructions. Gene expression was assayed using broad human genome specific HG-U133_Plus_2 GeneChip expression probe arrays (Affymetrix).