Anatomical diversity of dermis in homeostasis and wound repair

Skin has distinct characteristics depending on the anatomical site; however, the cell and molecular differences, and their functional implications, have been little described. RNA-sequencing of healthy adult mouse skin from the abdomen, back, and face/cheek has revealed that dermis from different sites is distinct, and that this aligns with their diverse embryonic origins (abdominal dermis develops from lateral plate mesoderm, dorsal dermis from paraxial mesoderm, and cheek dermis from neural crest). The functional implications for wound repair are evident from the differences in extracellular matrix and cell migration observed in tissue and dermal fibroblasts from these sites, and the histological and transcriptional variations during a wound response. 24 samples have been analysed by RNA-seq (total RNA): 4 mice for each of 3 anatomical sites (abdomen, back, cheek), and each mouse included a Day 3 full-thickness 2mm punch-biopsy skin wound and an adjacent unwounded "paired" control.皮肤的特征随解剖部位不同而存在显著差异，但目前针对不同部位皮肤的细胞与分子差异及其功能意义的相关研究鲜有报道。本研究对来自腹部、背部及面部/颊部的健康成年小鼠皮肤开展RNA测序（RNA-seq）后发现，不同解剖部位的真皮具有明显异质性，且该差异与其各异的胚胎发育起源高度吻合：腹部真皮源自侧板中胚层，背部真皮源自轴旁中胚层，颊部真皮则起源于神经嵴。不同部位皮肤的细胞外基质、真皮成纤维细胞(dermal fibroblasts)的细胞迁移特性差异，以及创伤应答过程中的组织学与转录组变化，均印证了其在伤口修复中的功能意义差异。本数据集共通过总RNA的RNA测序分析了24份样本：3个解剖部位（腹部、背部、颊部）各选取4只小鼠，每只小鼠均包含第3天的全层2mm穿刺活检皮肤伤口样本，以及其相邻的未损伤配对对照样本。