High-throughput sequencing of mRNA from oocyte, 1 cell, 16/32 cells, 128/256 cells, 3.5hpf and 5.3hpf zebrafish embryos (Wild type; AB line)

mRNA seq based approach to determine the transcriptome dynamics during early development To study the mechanisms regulating this developmental event in zebrafish, we applied RNA deep sequencing technology and generated comprehensive transcriptome profiles of 6 developmental stages from oocyte to early gastrulation. We determined the expression levels of maternal and zygotic transcripts and clustered them based on expression pattern. We identified a large number of novel transcribed regions in un-annotated regions of the genome, as well as splice variants with an estimated frequency of 40-75% during early zebrafish embryogenesis. Our data constitute a useful resource for developmental studies, gene discovery, and genome annotation.基于mRNA测序（mRNA sequencing）的早期发育转录组动态解析方法 为探究斑马鱼发育事件的调控机制，本研究采用RNA深度测序（RNA deep sequencing）技术，构建了从卵母细胞（oocyte）至早期原肠胚形成（early gastrulation）的6个发育阶段的全面转录组图谱。我们定量测定了母源转录本与合子转录本的表达水平，并依据表达模式对其开展聚类分析。本研究在基因组未注释区域中鉴定出大量全新转录区域，同时估算得到斑马鱼早期胚胎发生（embryogenesis）过程中可变剪接变体（splice variant）的发生频率为40%~75%。本数据集可为发育生物学研究、基因发现及基因组注释提供极具价值的参考资源。