mRNA profiling at various time points during adipocyte differentiation of human Multipotent Adipose-Derived Stem (hMADS) cells. Homo sapiens

human Multipotent Adipose-Derived Stem (hMADS) cells constitute a highly interesting in vitro model to study adipocyte development in vitro. Using a chemically defined medium with several hormonal inducers, these cells can be differentiated into mature adipocytes within ~2 weeks. To gain a global view on transcripts which are induced or downregulated at various stages of adipocyte differentiation, we performed a microarray analysis with samples harvested at the stem cell stage, the terminally differentiated stage, as well as various time points in between. Overall design: Analysis used proliferating hMADS cells as control sample for comparison to hMADS cells at confluence and at various time points after induction of adipocyte differentiation. Biological replicates: 2, indepently grown and harvested. All hybridizations were repeated with reversed dye assignment (dye-swap) as technical replicates.