Metatranscriptomics of Salmonella superspeader stool

Longitudinal analysis of Salmonella typhimurium mRNA from superspeader mouse cecal content and stool compared to in vitro Salmonella typhimurium mRNA. Overall design: Mice were infected with 10^8 colony forming units of Salmonella Typhimurium. 3 mice were euthanized at day 14 and 21 post-infection. Cecal content and feces were collected. RNA was extracted from those cecal contents and feces. In addition, salmonella was grown in LB for 10-16 hours aerobically and anaerobically for RNA extraction. In vivo and in vitro samples were sequenced and differential expression analysis was performed comparing the two sample groups.