Transcriptome analysis of MET-constitutively active versus MET-silenced colorectal cancer cells

To investigate the expression patterns driven by hyperactivated MET in MET-dependent cells, we employed LoVo colon adenocarcinoma cell line, harbouring a post-translational modification of MET that leads to its constitutive activation. We then silenced MET in LoVo cells using CRISPR/Cas9 knock-out (KO) protocol, and performed gene expression profiling analysis using data obtained from RNA-seq of cells with hyperactivated-MET versus MET-KO. Overall design: Comparative gene expression profiling analysis of RNA-seq data for LoVo cells and its KO derivative (LoVo MET KO)