Translating ribosome affinity purification of Esr1+ neuronal populations to identify sex and estrus-stage differentially expressed genes

We used translating ribosome affinity purification (TRAPseq) to profile genetically labeled neurons in the bed nucleus of the stria terminalis (BNST), preoptic area (POA), medial amygdala (MeA) and ventromedial hypothalamus (VMH) from adult male mice, and from adult female mice modeled to be in two distinct stages of the estrus cycle (estrus, sexually receptive, FR; and diestrues, sexually unreceptive, FNR). Overall design: We analyzed 36 bulk RNA-seq samples (each pooled from 3-5 mice) with three biological replicates per region and condition. Full DESeq2 output is available for each pairwise comparison.