Long-read sequencing using Arabidopsis T87 cultured cell under normal conditions

In gene expression, DNA is transcribed to mRNA, and mRNA is translated to protein. The transcribed RNA has various variants from a single gene, depending on the transcriptional start site, polyA addition site, and splicing pattern. As for the understanding of the gene expression process, accurate sequences of the entire length of the expressed RNA and comprehensive information on the amount of RNA is required. However, such analysis has been difficult to perform with conventional RNA-seq using short-read, next-generation sequencers. Thus, we conducted Nnopore sequencing using 3 day after inoculation of Arabidopsis T87 cultured cell under normal condition to comprehensively acquire the entire length of RNA sequence.