RNA sequencing of bone marrow hematopoietic progenitor and Gr1+/Mac1+ cells derived from Diap1 wild type, heterozygous, and knockout mice.

The goal of the experiment was to study the aberrant signalling pathways due to loss of or haploinsufficiency of Diap1 protein. We found that LPS receptor transcript is highly expressed in Diap1 deficient Gr1+/Mac1+ cells but not in Lin- cells, suggesting a role for Diap1 in determining the normal morphology of committed Gr1+/Mac1+ cells. Examination of gene expression profiles of bone marrow hematopoietic progenitor and Gr1+/Mac1+ myeloid cells from Diap1 wild type, heterozygous, and knockout mice.本实验旨在探究Diap1蛋白缺失或单倍体剂量不足（haploinsufficiency）所引发的异常信号通路。研究发现，脂多糖（LPS）受体转录本在Diap1缺陷的Gr1+/Mac1+细胞中呈高表达状态，而在谱系阴性（Lin-）细胞中无此特征，这提示Diap1在维持定型分化的Gr1+/Mac1+细胞的正常形态中具有重要作用。本研究对来自Diap1野生型、杂合子及敲除型小鼠的骨髓造血祖细胞与Gr1+/Mac1+髓系细胞的基因表达谱展开了检测分析。