IC50 values of the diminazene aceturate drug evaluated for B. bovis and T. equi with and without daily replacement of the medium using the fluorescence-based method.

a IC50 values for diminazene aceturate were determined on the fourth day of in vitro culture using the fluorescence-based method with 2.5% and 5% HCTs for B. bovis and T. equi, respectively, in three separate experiments. Each drug concentration was made in triplicate in each experiment, and the final obtained IC50s were the mean of three obtained values from separate experiments.b The differences between IC50 values determined using the fluorescence-based method with and without daily replacement of the medium were not statistically significant.IC50 values of the diminazene aceturate drug evaluated for B. bovis and T. equi with and without daily replacement of the medium using the fluorescence-based method.