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GENE TARGETING IN PLANTS USING A CHIMERIC MULTI DOMAIN RECOMBINATION PROTEIN

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国家林业和草原科学数据中心2023-02-12 更新2024-03-07 收录
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An isolated polynucleotide sequence that encodes for a multi domain recombination protein, comprising a donor DNA-Binding domain (BDB); and a chromosomal binding domain (CBD). A plant cell can be transformed by introducing into the cell a first nucleic acid sequence, which comprises a plant nuclear promoter operably linked to a first nucleic acid sequence comprising the stated polynucleotide sequence, and a second nucleic acid sequence, which comprises a donor nucleic acid sequence and at least two flanking sequences capable of binding to a donor binding domain of the protein, the sequences being located adjacent to each end of the donor polynucleotide sequence forming left and right borders thereto.
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国家林业和草原科学数据中心
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2023-02-12
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