Evaluating bias-reducing protocols for RNA sequencing library preparation

ChEBI2014-06-24 更新2026-07-21 收录

RNA测序文库制备

转录组学实验优化

数据链接：

https://www.ebi.ac.uk/biostudies/ArrayExpress/studies/E-MTAB-2566 数据链接链接失效反馈

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资源简介：

Evaluating bias-reducing protocols for RNA sequencing library preparation

创建时间：

2014-06-24

Analysis of SCOTS effect on transcript population

转录组学实验优化

SCOTS技术评估

The cDNA obtained before and after each round of SCOTS (first, second and third) from 2 h post-infection were hybridized to analyse the effect of SCOTS round on transcripts population. The number of d

NIAID Data Ecosystem80

Systematic evaluation of RNA-Seq preparation protocol performance (RNASeq: TruSeq)

RNA测序文库制备

差异表达分析

In this study, we present a comprehensive evaluation of four RNA-Seq library preparation methods. We used three standard input protocols, the Illumina TruSeq Stranded Total RNA and TruSeq Stranded mRN

NIAID Data Ecosystem50

The effect of metal cations on the capture of Pol II-derived nascent transcripts in NET-seq experiments. The effect of metal cations on the capture of Pol II-derived nascent transcripts in NET-seq experiments

转录组学实验优化

PolII占据分析

The goal of this study was to test the effect of metal cations (CaCl2 and MnCl2) during Pol II NET-seq. These metals are readily used in the majority of studies that use NET-seq to analyze Pol II occu

NIAID Data Ecosystem20

Supplemental Material for Pallares, Picard, and Ayroles, 2020

RNA测序文库制备

转录组技术

File S1 contains step-by-step instructions for the TM3’seq library preparation protocol. File S2 contains step-by-step instructions for the mRNA extraction protocol. File S3 describes how to implement

DataCite Commons2020-07-14 更新60

Impacts of quality filtering and rRNA depletion on resultant RNA-Seq dataset sizes.

RNA-seq数据预处理

转录组学实验优化

Impacts of quality filtering and rRNA depletion on resultant RNA-Seq dataset sizes.

NIAID Data Ecosystem30