Quantitative analysis of mouse Dpl, PrP and Sho interactomes identifies metal ion transporters of the ZIP protein family in spatial proximity to all three members of the mammalian prion protein family.

aProteins were sorted into specific versus unspecific binder categories based on their iTRAQ distribution, i.e. proteins were considered unspecific interactors if their derived CID spectra revealed iTRAQ114 to 117 signature mass peak signal intensities which exceeded 10% of combined intensities for all samples including the unspecific control.
bFor the calculation of iTRAQ values the intensity of individual peptide associated iTRAQ signature peaks was normalized to combine to 100% per peptide and subsequently averaged. Standard deviations were determined and are listed in Table S1.
cLength of precursor molecules prior to posttranslational processing.
dOnly CID spectra underlying different peptides were considered i.e. if the same peptide was identified with different charge states or modifications it counted as one hit.
eTotal number of unique CID spectra. Please note that the same peptide was only counted more than once if it was identified with different charge states or modifications.
fPercent sequence coverage based on the presence of peptides for which no higher ranked assignment to other proteins could be made.