Deciphering the endogenous cistrome and transcriptome of estrogen receptor beta in ovary

ERß is predominantly expressed in granulosa cells of the ovary, where its important for normal ovarian function and plays a vital role in folliculogenesis. However, the genome wide binding landscape of ERß in the ovary was not determined and its interaction targets are unknown. To characterize the mechanism of ERß, we performed ChIP-Seq using endogenous ERß expression and mapped the cistrome of ERß in the ovary using a validated antibody. Overall design: ChIP-Seq was performed three times in the ovaries endogenously expressing ERß. Binding sites present in at least two out of three replicates were considered for downstream analysis,