Gene expression at single cell level of stromal cells from the distal colon of normal sites of colorectal cancer and inflamed sites of ulcerative colitis.

Glial cells are considered as anti-inflammatory population in the lamina propria. We used single cell RNA sequencing (scRNA-seq) to analyze the diversity of stromal cells in the lamina propria of colon. Live CD90+CD45-CD31-EpCAM- stromal cells from human lamina propria mononuclear cells (LPMCs) of colorectal cancer and ulcerative colitis (UC) were isolated by Fluorescence activated cell sorting (FACS) and analysed using scRNA-seq.