Discovery of prolactin-like in lamprey: Role in osmoregulation and new insight into the evolution of the growth hormone/prolactin family

These data were generated to characterize prolactin-like (PRL-L) and growth hormone (GH) in sea lamprey (Petromyzon marinus) in hormone-receptor binding, tissue distribution, and gene expression patterns at various lifecycle stages, in metamorphosis, and in acclimations to hyperosmotic and hypoosmotic conditions, as well as to detect the hormonal effects on their signaling systems and branchial osmoregulation-related ion transporters. For the hormone-receptor binding assay, recombinant PRL-L and GH were radioactively labelled with 125iodine and applied for determination of binding affinities with recombinant sea lamprey GH receptor and PRL receptor expressed by HEK293 cell line. Relative abundance of each gene in the cDNA samples synthesized upon the RNAs of various tissues of sea lamprey was assessed using quantitative real-time PCR (qPCR). Semiquantification of immunoreactive staining in percent area of proximal pars distalis was conducted to compare the hormone levels in the pituitar...