Wheat plant inoculated with Puccinia striiformis f. sp. tritici

We sequenced two groups of wheat-rust interaction combination, named NIL_R vs. CYR32 and NIL_S vs. CYR32 and selected the time point at 0, 18, 24, 48, 96, and 168 hpi. The wheat cultivar NIL_R (Yr26) and NIL_S (yr26) were generated by 92R137 (Yr26 gene donor) backcross with recurrent parent Yangmai 158 for six times and self-cross for four times (BC6F4). A single-spore isolate of CYR32 was reproduced on seedlings of wheat cultivar Mingxian169. The fresh urediospores were collected and used for inoculating. NIL_S vs. 32R were compatible group (wheat is susceptible to the rust), while NIL_R vs. CYR32 was incompatible group (wheat is resistance to the rust). The compatible group and incompatible group were simply named 32S and 32R, respectively. Each sample was sequenced 10 Gb on HiSeq2500 (PE125), and mapped to Chinese spring (TGACv1) and CYR32 reference.