Espada_et_al_2025_RIP Western blots

The Western blots in the file are the original blots included in the Supplementary Figure S8. RNA Immunoprecipitation of proteins identified as potential lncRNAs interactors in S1m pull-down. Five proteins (Lb1, Lb2, Lb3, Lb4 and Lb5) were selected for RIP assays based on the S1m pull-down results. Immunoprecipitation was done using anti-HA magnetic beads and confirmed by western blotting using anti-HA antibody. Each membrane contains the total cell lysate, the input material (cell lysate after centrifugation for debris removal), the flow through (FT), the wash 1 (W1) and wash 7 (W7) and the eluates from the samples treated (Elution RNAse) or not (Elution) with RNAse.