Antitumor effects of DNA-RNA nanocarriers linked to AS1411 aptamer

To study the effectiveness of DNA-RNA molecules linked with aptamers as hybrid vectors to target tumor cells and introduce functional RNA molecules into cells, as well as their effects on tumor cells. Methods Design and synthesize short complementary DNA and RNA molecules, and assemble them into DNA-RNA hybrid chains; connect AS1411 aptamer as targeting molecule, and then connect p21 saRNA and TIGIT siRNA as drug molecules, denoted as P21 saRNA and TIGIT siRNA , constitute a hybrid vector, the general structural formula is AS1411-DNA/RNA-sxRNA; Detect whether AS1411-DNA/RNA-sxRNA can target and enter tumor cells and its effect on the survival, migration, invasion and apoptosis of tumor cells.In this study, a DNA RNA hybrid vector targeting AS1411 nucleic acid aptamer and carrying p21 saRNA or TIGIT siRNA nucleic acid drugs has been successfully constructed. At the same time, it has been endowed with targeting and drug functions. It successfully delivers target microRNAs into cells and regulates the expression of target genes, providing a new idea for the research and development of RNA delivery systems. As a new delivery strategy, DNA RNA hybrid vectors have good research and development value.This data set mainly includes the original data and relevant pictures of AS1411 hybrid carrier assembly, serum stability test, fluorescence microscope and cell function experiment involved in the paper