Supplementary materials sequences of soluble chemical communication proteins in Anagrus nilaparvatae

Anagrus nilaparvatae is an important egg parasitoid wasp of pests such as
the rice planthopper. Based on the powerful olfactory system of sensing
chemical information in nature, A. nilaparvatae shows complicated life
activities and behaviors, such as feeding, mating and hosting. We
constructed a full-length transcriptome library and used this to identify
the characteristics of soluble chemical communication proteins. We
analyzed the sequence characteristics of soluble chemical communication
protein genes and identified eight genes: AnilOBP2, AnilOBP9, AnilOBP23,
AnilOBP56, AnilOBP83, AnilCSP5, AnilCSP6 and AnilNPC2. After sequence
alignment and conserved domain prediction, the eight proteins encoded by
the eight genes above were found to be consistent with the typical
characteristics of odorant-binding proteins (OBPs), chemosensory proteins
(CSPs) and Niemann-pick type C2 proteins (NPC2s) in other insects.
Phylogenetic tree analysis showed that the eight genes share low homology
with other species of Hymenoptera. This study provides the first data for
A. nilaparvatae’s molecular characteristics of soluble chemical
communication proteins, as well as an opportunity for understanding how A.
nilaparvatae behaviors are mediated via soluble chemical communication
proteins.