Escherichia coli str. K-12 substr. MG1655 Genome sequencing. Escherichia coli str. K-12 substr. MG1655

C321.deltaA was adapted over ~1,100 generations to fast growth in M9 minimal media supplemented with 1% glucose. In so doing, many off-target mutations leftover from the original recoding efforts were corrected as either direct reversions, or more often compensatory adaptive mutations. Additionally, mutations known to be endemic to all MG1655-derived strains, including the mutation to rph which causes pyrimidine starvation in minimal media, were corrected or compensated for. We present here the genome of a recoded C321 strain with no TAG codons adapted for fast growth in M9 and other defined media.