RIP-Seq analysis of Trypanosoma brucei ELAV-like protein Tb927.8.6650
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https://www.ncbi.nlm.nih.gov/sra/SRP021144
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资源简介:
A tagged ectopic version of the ELAV-like protein Tb927.8.6650 of T. brucei was expressed in stable cell lines and pulled down. Co-purifying transcripts were analyzed by sequencing to identify RNAs associated with Tb927.8.6650. Overall design: Stable procyclic form cell lines expressing tetracycline-inducible TAP-tagged Tb927.8.6650 were created. Cells were harvested 48h after tet-induction, followed by tandem affinity purification of Tb927.8.6650, extraction of co-purified RNA, and sequencing.
创建时间:
2019-09-23



