H3K9me2 profile of tumor infiltrated Treg from the WT and the jmjd1c TregcKO mice and chromatin accessibility profile of tumor treg and spleen treg

Tumor infiltrated treg cells are considered as a crucial in the cancer immunotherapy. We used H3K9me2 CUT&RUN to analyze the differences between WT and jmjd1cTreg cko mice and clarify the important function of jmjd1c in tumor treg. We use ATACseq to analyze the difference between tumor treg and spleen treg and characterize the epigenetic characters in tumor treg. Overall design: Tumor infiltrated treg of the WT and jmjd1c tregcKO mice were isolated by Fluorescence-activated cell sorting (FACS) according to the presence or absence of fluorence signal and analyzed using H3K9me2 CUT&RUN. Tumor infiltrated treg and spleen treg were isolated by Fluorescence-activated cell sorting (FACS) according to the presence or absence of fluorence signal and analyzed using ATACseq. Tumor infiltrated treg of the WT and jmjd1c tregcKO mice were isolated by Fluorescence-activated cell sorting (FACS) according to the presence or absence of fluorence signal and analyzed using and H3K9me2 CUT&RUN.