E. coli Gyrase Slimfield Microscopy Dataset

# Project: E. coli Gyrase strain AS912 Slimfield Microsocopy # Dataset Description This archive contains processed tracking data from a series of Slimfield microscopy and FRAP experiments performed to analyse DNA Gyrase in live E. coli cells. ## Folder Structure The datasets are organised hierarchically as follows: ```Experiment_folder/├── field1/│ ├── tracks.mat├── field2/│ ├── tracks.mat├── ...``` Each **experiment folder** contains multiple **field folders**. Each **field folder** contains a `tracks.mat`: Tracking data for that field of view. ## Data Overview - Each experiment corresponds to an independent biological or imaging experiment.- Each field represents a separate imaging region within an experiment.- Tracking was performed independently for each field. ## Analysis Code The MATLAB analysis code used to process and analyse these data is available in a separate Zenodo repository: https://doi.org/10.5281/zenodo.15805285 This code repository contains the full analysis pipeline used across multiple related projects. ## FAIR Data Statement This dataset has been prepared according to FAIR principles:- **Findable**: Deposited in Zenodo with a persistent DOI.- **Accessible**: Available as a downloadable archive.- **Interoperable**: Provided in standard MATLAB `.mat` format.- **Reusable**: Includes structured organisation and accompanying code. ## Notes - All data are provided in MATLAB `.mat` format.- Folder names correspond to experiment and field identifiers used during acquisition and analysis.- Users should consult the associated code repository for details on loading and processing the data. ## Contact For questions regarding this dataset, please contact the corresponding author.