Jennifer Bestman, Holly Cline (2011) CIL:37103, Xenopus laevis, CNS neuron (sensu Vertebrata). CIL. Dataset

This is IMAGE #3 (day 3) of a developing neuron from the optic tectum of an albino Xenopus laevis tadpole CNS, stage 47.
The cell was imaged daily over 3 days and shows the development of the dendritic arbor over time.

The cell was transfected with in vivo electroporation of plasmid DNA encoding eGFP ~24 hours before the image was acquired. The intact, living tadpole was anesthetized, positioned under a glass coverslip and this image was taken directly through its transparent skin using a custom built two-photon microscope.

此为第3日（第3天）白眼斑蝶螈（Xenopus laevis）中枢神经系统视顶盖中发育中的神经元的图像#3。该细胞在连续3天内每日进行成像，展现了树突丛随时间的发展过程。在图像获取前约24小时，通过体内电穿孔法将该细胞转染编码绿色荧光蛋白（eGFP）的质粒DNA。实验中，完整的活体蝌蚪经过麻醉处理，置于载玻片下，并直接通过其透明皮肤使用定制建造的双光子显微镜进行成像。