Kinetic parameters of amyloid formation from metalated and apo-SOD1 in 1 M guanidine.

SOD1 was incubated in buffers containing 50 mM citrate pH 3, formate pH 4, acetate pH 5, succinate pH 6, or MOPS pH 7, plus 0.1 M NaCl and 1 M guanidine. The time course of ThT fluorescence was fit to a sigmoidal equation and the amplitude and lag time determined as described in “Materials and Methods”. Dashes indicate that no amyloid was formed.