The Tug1 locus is required for male fertility and comprised of DNA, RNA and Protein regulatory elements

We have sequenced mouse embryonic fibroblasts (MEFs) and 6 organs (testes, prostate, liver, heart, spleen, and eye) harvested from adult male wild type and Tug1 knockout (Tug1_tm1.1Vlcg) mice in a C57BL/6J/129S6 (N3-C57BL/6J, no Neo) background. Additional sequencing was done on the testes of a Tug1 rescue mouse, which contains a doxycycline inducible allele for Tug1 (tg(Tug1)) and CAGs-rtTA3 in the Tug1 knockout background (Tug1_tmn1.1Vlcg). Comparison between wild type and Tug1 knockout (Tug1_tm1.1Vlcg) organs and primary cell transcriptomes. Additional comparisons of the transcriptomes of wildtype, knockout, and rescue testes.本研究对C57BL/6J/129S6（N3回交至C57BL/6J品系，无新霉素抗性标记）背景下的成年雄性野生型及Tug1基因敲除（Tug1_tm1.1Vlcg）小鼠的小鼠胚胎成纤维细胞（mouse embryonic fibroblasts, MEFs），以及6种器官（睾丸、前列腺、肝脏、心脏、脾脏与眼睛）进行了转录组测序。此外，本研究还对Tug1挽救小鼠的睾丸开展了额外测序：该挽救小鼠处于Tug1敲除背景（Tug1_tmn1.1Vlcg）中，携带强力霉素诱导型Tug1等位基因（tg(Tug1)）与CAGs-rtTA3元件。本数据集包含两类对比分析：一是野生型与Tug1敲除（Tug1_tm1.1Vlcg）小鼠的器官及原代细胞转录组对比，二是野生型、敲除型及挽救型小鼠的睾丸转录组对比。