Protect and shield strategy of reactive acyl glucuronides licenses precision-omics investigations into drug-induced liver injury

RNA-Seq Profiling of SILAC-G-REX Platform (Transfected HEK-293) Exposed To Reactive Acyl-Glucuronides Overall design: HEK293T cells ectopically expressing Halo were treated with either Ht-Pre-AG-ibufenac or Ht-Pre-AG-ibuprofen for 2 h (10 µM). Following washout cycles to remove excess non-Halo-bound probes, cells were exposed to light (3 min, 365 nm, 5 mW/cm2), to induce rapid liberation of respective AG-drugs and transcriptomics changes were assessed.