Sox10 Binding Analysis in the Central and Peripheral Nervous System

ChIP-seq of Sox10 in spinal cord and sciatic nerve was performed to determine shared and unique binding sites for Sox10 in oligodendrocytes and Schwann cells, respectively. Sox10 is required for both Schwann cell and oligodendrocyte development. In addition, differentiation of myelinating glia is dependent upon axonal signaling, so these studies were performed in vivo.本研究针对脊髓（spinal cord）与坐骨神经（sciatic nerve）中的Sox10开展染色质免疫共沉淀测序（Chromatin Immunoprecipitation sequencing，ChIP-seq），旨在分别鉴定少突胶质细胞（oligodendrocytes）与施万细胞（Schwann cells）中Sox10的共有结合位点与特异性结合位点。Sox10对于施万细胞和少突胶质细胞的发育均不可或缺。此外，髓鞘形成胶质细胞（myelinating glia）的分化依赖于轴突信号传导，因此本研究在活体（in vivo）环境中开展。