Effects on gene expression by Aff3-deficiency. Effects on gene expression by Aff3-deficiency
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To analyze the effect of Aff3-deficeincy on gene expression in B cells, we employed cDNA microarray analysis. The data indicated that the deficiency altered the expressions of very limited number of genes. Overall design: CD43-negative B cells were purified from Aff3+/- or Aff3-/- mouse spleen, and cultured in the presence of 10 ug/mL LPS and 10 ng/mL IL-4 for 3 days, and total RNA was extracted from these cells and used for the analysis.
创建时间：
2022-01-14
