Rat transcriptomic profiling of liver sinusoidal endothelial cells during chronic liver disease reveals stage-specific secretory signature

RNAseq was applied to define the transcriptome of primary LSECs from rats with acute liver injury, mild liver fibrosis and advanced chronic liver disease (CLD) (due to CCl4 or thioacetamide administration). Results showed different transcriptomic profile during progression of CLD, and a large number of significant de-regulated genes (1412) were shared in cirrhotic CCl4- and TAA-LSECs (fold-change>1.5 and p-vale<0.05)compared with their respective healthy-LSECs.本研究采用RNA测序（RNA Sequencing，RNAseq）技术，对急性肝损伤、轻度肝纤维化及晚期慢性肝病（chronic liver disease，CLD）模型大鼠的原代肝窦内皮细胞（Liver Sinusoidal Endothelial Cells，LSECs）开展转录组分析。该慢性肝病模型通过给予四氯化碳（carbon tetrachloride，CCl4）或硫代乙酰胺（thioacetamide，TAA）诱导构建。结果表明，慢性肝病进展过程中，转录组特征存在显著差异；与各自对应的健康对照组肝窦内皮细胞相比，四氯化碳造模及硫代乙酰胺造模的肝硬化组肝窦内皮细胞中共存在1412个共享的显著差异调控基因（倍数变化>1.5且P值<0.05）。