High-Throughput Sequencing Analysis of miRNAs in Pancreatic Ductal Adenocarcinoma

This study aimed to investigate the miRNA expression profiles in pancreatic ductal adenocarcinoma (PDAC) to identify novel potential diagnostic markers and therapeutic targets. Using high-throughput sequencing technology, we analyzed miRNA expression levels in 3 PDAC tissues with good prognosis (T1-T3) and 3 PDAC tissues with poor prognosis (T4-T6). The results revealed significantly distinct miRNA expression patterns between the two groups. Our study identified prognosis-associated miRNAs in PDAC and evaluated their potential as therapeutic targets. Overall design: To systematically identify potential prognostic miRNA biomarkers, this study collected PDAC tissue samples from patients with good (T1–T3) and poor (T4–T6) prognoses. After total RNA extraction, high-throughput sequencing was performed to comprehensively profile differentially expressed miRNAs between the two groups.