Risk of parasiticides diflubenzuron, eprinomectin, and fenbendazole to dung-associated beetles

Dung-associated beetles provide crucial ecosystem services, including reducing pasture fouling and contributing to animal and pasture health. However, parasiticides used on cattle can negatively impact beetles. We assessed the exposure and impact of Clarifly ® (active ingredient [a.i.] diflubenzuron), LongRange ® (a.i. eprinomectin), and Safe-guard ® (a.i. fenbendazole) on beetles and flies. Using high-performance liquid chromatography, we quantified the residual concentrations of these chemicals in cattle feces for 150 days. We found fenbendazole excreted within the first 3 days after treatment (DAT), while diflubenzuron and eprinomectin persisted for 8-12 weeks. To estimate the concentrations of these active ingredients that are toxic to beetles, we dosed cattle dung with diflubenzuron, eprinomectin, or fenbendazole, allowed insects to colonize in the field, and monitored the emergence of beetles. Dung beetle (Scarabaeidae) abundance was ne..., , # Risk of parasiticides diflubenzuron, eprinomectin, and fenbendazole to dung-associated beetles

Dataset DOI: [10.5061/dryad.8kprr4z06](10.5061/dryad.8kprr4z06)

## Description of the data and file structure

We assessed the exposure and impact of Clarifly® (active ingredient [a.i.] diflubenzuron), LongRange® (a.i. eprinomectin), and Safe-guard® (a.i. fenbendazole) on beetles and flies. Using high-performance liquid chromatography, we quantified the residual concentrations of these chemicals in cattle feces for 150 days.

To estimate the concentrations of these active ingredients that are toxic to beetles, we dosed cattle dung with diflubenzuron, eprinomectin, or fenbendazole, allowed insects to colonize in the field, and monitored the emergence of beetles. 

### Files and variables

#### File: HPLC_Data.csv

**Description:** Contains concentrations of parasiticides diflubenzuron, fenbendazole, eprinomectin, ivermectin and moxidectin from samples in 2022 and 2023. We did not include re...,