RNA-seq of Bacillus subtilis WT W168 treated with the SynAnt 49 (BDTL049) (4 µg ml-1) compared to untreated control

To obtain global cellular response of Bacillus subtilis WT W168 when treated with SynAnt 49 (BDTL049) we performed RNA-seq experiments. For this, we added 4 µg ml-1 SynAnt 49 to logarithmic phase growing cells and harvested cells after 10 min exposure. Experiments were performed in triplicates and non-induced Bacillus subtilis WT W168 was used as reference condition. Examination of SynAnt 49 (BDTL049) induced Bacillus subtilis WT cells compared to non-induced Bacillus subtilis WT cells Please note that [1] the 'tm5_2_05_*_no_treatment' raw data (i.e. GSM4485628- GSM4485630 in GSE148903) was re-analyzed for the current study (as reference sample data) and duplicated sample records were created for the convenient retrieval of the complete raw data from SRA [2] the counts*.txt files both contain the counts of the reference (TM5_2_05_1-3) compared to AmpB (TM5_2_23_1-3; GSE148903), BDTL049 (TM5_2_24_1-3; current study) and some Streptomyces supernatants (TM5_2_25_1-3; submission in preparation). These analysis were pooled in the respective files to increase the reproducibility of the DESeq2 analysis.