SEED SORTING METHOD FOR FIXING PLANT HETEROSIS
收藏国家林业和草原科学数据中心2023-02-12 更新2024-03-07 收录
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资源简介：
Provided is a method for sorting cloned seeds using a pollen specific gene switching system. Three closely linked gene expression cassettes and a MiMe knockout vector that knocks out three key meiotic genes， REC8， PAIR1 and OSD1， are simultaneously transferred into a hybrid plant. The three gene expression cassettes comprise: 1) an embryonic autonomous gene expression cassette E1; 2) a screening marker gene expression cassette E2， which is regulated by E3; and 3) an expression cassette E3 for pollen-specific regulation of E2. The screening marker regulated by the pollen specific genetic switching system is used to sort asexual embryo seeds and zygotic embryo seeds from the self-fruiting seeds of hybrid transgenic plants. The cloned seeds retain heterosis and can be used for production， and the remaining seeds can be used for commercial purposes.
提供机构：
国家林业和草原科学数据中心
创建时间：
2023-02-12
