Whole transcriptome analysis in 661W photoreceptor cells

This experiment aimed to characterise the complete transcriptional profile of 661W cells to identify their origin as cone photoreceptor cells. Cells were either grown in complete media or subjected to 72 hours of serum starvation to encourage differentiation. Overall design: 661W cells were either grown in normal complete medium containing serum, or exposed to 72 hours of serum starvation. This was repeated across 3 independent experiments, with cells of passage number 24, 26 and 27. Total RNA was extracted using TRI reagent, samples treated with Dnase, and cleaned on an RNA clean and concentrate column.RNA-seq was performed on an Illumina NextSeq 500 instrument with six libraries multiplexed and run across 4 lanes per flow-cell using 75 bp single end reads in high output mode.