Supplementary Dataset S6. AvrRpm1 ADP-ribosylates PLDGAMMA3 when expressed in N. benthamiana.

During infection, the plant pathogenic bacterium Pseudomonas syringae translocates type 3 effector proteins into the host cell. The effector protein AvrRpm1 is an ADP-ribosyltransferases that ADP-ribosylates host proteins to subvert plant innate immunity. The aim of this experiment was to identify AvrRpm1-mediated ADP-ribosylation sites on Arabidopsis AT4G11840 (PLDGAMMA3) by co-expression in Nicotiana benthamiana. The affinity enrichment was performed using the enhanced Af1521 Macro domain versus a G42E mutant variant as control.